Human neutrophil gelatinase is a component of specific granules.
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چکیده
منابع مشابه
Human neutrophil gelatinase is a component of specific granules.
Previous investigators have proposed that gelatinase, a metalloproteinase found in neutrophils, is stored in a novel secretory compartment distinct from the two major granule populations, azurophilic and specific. To locate this proteinase in human neutrophils we reacted the cells for peroxidase and then applied monospecific polyclonal antibodies to human neutrophil gelatinase to immunolabel ul...
متن کاملHuman Neutrophil Granules and Secretory Vesicles Azurophil Granules Specific Granules Gelatinase Granules Secretory
neity simply that some proteins must be segregated to survive P inside the neutrophil, or for the neutrophil to survive, much OLYMORPHONUCLEAR leukocytes were discovered by Paul Ehrlich, when fixation and staining techniques as two-component glue has to be stored in two separate made it possible to identify the lobulated nucleus and the tubes, although both will be used at the same time? Whethe...
متن کاملSubcellular localization and release of human neutrophil gelatinase, confirming the existence of separate gelatinase-containing granules.
An e.l.i.s.a. was developed using specific polyclonal rabbit antibodies against human neutrophil gelatinase. This assay, in contrast to the functional assay, is independent of activation of gelatinase, and is specific for the detection of gelatinase in both its reduced and unreduced forms. Using this assay, we were able to demonstrate a difference between the subcellular localization of gelatin...
متن کاملSpecific Granules of Human Neutrophil Leucocytes
many tissues, but are not usually stored intracellularly in appreciable amounts. Polymorphonuclear neutrophil leucocytes are a notable exception. In these cells collagenase has been found in the granule fraction (Lazarus et al., 1968, 1972), and, like other granule components (Bainton et al., 1971), is presumably synthesized and packaged into granules during maturation. Two main types of granul...
متن کاملIdentification of Neutrophil Gelatinase-Associated Lipocalin as Novel Matrix Protein of Specific Granules
Neutrophil gelatinase-associated lipocalin (NGAL) is a novel 25-kD protein of human neutrophils, that is in part covalently complexed with neutrophil gelatinase. However, both NGAL and gelatinase exist mainly in forms not associated with each other. An explanation for this phenomenon might be that the unassociated proteins reside in different subcellular compartments. The aim of the present stu...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1989
ISSN: 0021-9738
DOI: 10.1172/jci114312